Lactic and succinic acids as excretory products of Polymorphus minutus (Acanthocephala) in vitro.
نویسندگان
چکیده
When adult worms of the acanthocephalan parasite, Polymorphus minutus, are incubated for 4 hr. at 417 ±0-5° C. under near-anaerobic conditions in a balanced salt solution containing radioactive glucose, they excrete two substances which contain approximately equal amounts of radioactivity. It was suspected that these metabolites might be organic acids. They were found to be non-volatile, dialysable, free from phosphorus and amino groups and not carbohydrate in nature. Examination of the incubation medium by one-dimensional, descending paper chromatography in a Shandon Panglass 300 Chromatatank indicated that one of the metabolites was lactic acid. The solvent systems used were PrF and EtAm (Smith, i960), and the organic acids were located by dipping dry papers in the bromcresol green reagent described by Smith. The other metabolite appeared to be succinic acid with the PrF solvent system, but its position on the paper after the EtAm solvent did not correspond exactly with that of authentic succinic acid, although it was always in the same region of the paper as succinic, malic and fumaric acids. The aim, therefore, of the work described in this paper was to identify, by means of gas chromatography, the unknown organic acid and to confirm the identity of the lactic acid. The results are considered to be applicable to the carbohydrate metabolism of the worms in vivo, because care was taken to ensure that the worms remained healthy during the incubation period.
منابع مشابه
Studies on the absorption and metabolism of D-(u-14C) glucose by Polymorphus minutus (Acanthocephala) in vitro.
متن کامل
Lipid, bile acid, water and dry matter content of the intestinal tract of domestic ducks with reference to the habitat of Polymorphus minutus (Acanthocephala).
The data presented in this paper were obtained to define more accurately the environment of Polymorphus minutus, an acanthocephalan parasite of ducks. This parasite inhabits a region of the duck's intestine extending from 60% to 80% of the intestinal length (Crompton & Whitfield, 1968). In the case of Momliformis dubius, an acanthocephalan parasite of rats, Edmonds (1965) found that nutrients a...
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Most individuals in an infection of the acanthocephalan parasite Polymorphus minutus (Goeze, 1782) in domestic ducks are found in a region extending from 65% to 75% of the distance along the host's intestine (Crompton & Harrison, 1965). The exact location and mode of attachment of the parasites in this habitat has been described by Crompton, Shrimpton & Silver (1965). Acanthocephalans, like ces...
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ورودعنوان ژورنال:
- The Journal of experimental biology
دوره 46 3 شماره
صفحات -
تاریخ انتشار 1967